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KMID : 0624620110440070446
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BMB Reports
2011 Volume.44 No. 7 p.446 ~ p.451
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Combination of Runx2 and BMP2 increases conversion of human ligamentum flavum cells into osteoblastic cells
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Kim Hyun-Nam
Min Woo-Kie
Jeong Jae-Hwan
Kim Seong-Gon
Kim Jae-Ryong
Kim Shin-Yoon
Choi Je-Yong
Park Byung-Chul
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Abstract
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The conversion of fibroblasts into osteoblasts requires the activation of key signaling pathways, including the BMP pathway. Although Runx2 is known to be a component of the BMP pathway, the combination of Runx2 and BMP2 has not yet been examined with respect to the conversion of fibroblasts into osteoblasts. Here, human ligamentum flavum (LF) fibroblast- like cells from six patients were tested for their conversion into osteoblasts using adenoviruses expressing Runx2 or BMP2. The forced expression of Runx2 or BMP2 in primary cultured LF cells resulted in a variety of proliferation and differentiation behaviors. Combined treatment of BMP2 plus Runx2 resulted in better osteoblastic differentiation than treatment with either component alone. These results indicate that the Runx2 and BMP2 pathways possess both common and independent target genes. Collectively, Runx2 plus BMP2 mediated efficient conversion of fibroblast-like LF cells into osteoblast- like cells, suggesting the possible use of these components for clinical applications such as spinal fusion.
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KEYWORD
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BMP2, Conversion, Ligamentum flavum cells, Runx2
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